endonuclease
英[endə'njuːklɪeɪs]
美[ˌendoʊ'njuːkliːˌeɪs]
- n. [生化]核酸内切酶
详细释义
Noun:
-
a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes
双语例句
- 1. PCR products were digested with endonuclease and sequenced.
- PCR产物经限制性内切酶消化、序列测定及分析。
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- 2. Methods:DNA sequencing and restriction endonuclease reaction was used.
- 方法：采用DNA测序及限制性酶切反应方法。
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- 3. The structure of vector was identified by endonuclease analyzing and base sequencing.
- 通过核酸内切酶酶切分析及测序鉴定载体结构。
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- 4. After a restriction endonuclease cleavage, two expected smaller fragments were observed.
- 经限制性内切酶反应，获得两个预期大小的片段。
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- 5. The core technology in TILLING is screening mutant library by using single-strand endonuclease.
- TILLING技术的核心是使用单链核酸内切酶大规模筛选突变体库。
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- 6. The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.
- 建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。
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- 7. Then, we analyzed the specificity of PCR primers through digesting PCR products with restriction endonuclease.
- 然后对扩增产物进行限制性酶切反应；
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- 8. In this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis.
- 本研究利用多种组合的酶切分析，绘制了较为详细的酶切图谱。
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- 9. Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the AGAR gel electrophoresis.
- 最后用限制性内切酶单酶切及琼脂凝胶电泳进行鉴定。
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- 10. The products of endonuclease digestion were run on 8% non-denaturing polyacrylamide gel electrophoresis and detected by silver staining.
- 用8%非变性聚丙烯酰胺凝胶电泳将酶切产物分离，并用银染法显色。
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- 11. Restriction enzyme: Protein (more specifically, an endonuclease) produced by bacteria that cleaves DNA at specific sites along its length.
- 限制性内切：由细菌产生的一种蛋白质，能在特定的地方切断去氧核糖核酸分子。收藏。
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- 12. Result Restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of OSCP gene can be successful.
- 结果酶切及测序显示对OSCP基因有义突变部分的纠正获得成功。
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- 13. Results: the constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.
- 结果经酶切和测序鉴定表明所构建的重组表达质粒中含有TSO45 - 4b基因。
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- 14. Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.
- 结果经pcr方法、酶切鉴定及直接测序鉴定出YMDD、YVDD和YIDD质粒构建成功，且该方法具有较好的敏感性和特异性。
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- 15. Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls;
- 并对发现突变的基因进行分析。方法应用PCR扩增、DNA序列测定等技术分析所有AR基因外显子及其邻近DNA序列片段；
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- 16. Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.
- 方法用盐提取法提取人群中白细胞的DNA，以聚合酶链反应、限制性内切核酸酶检测-4533G/A多态性。
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- 17. Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.
- 方法用盐提取法提取人群中白细胞的DNA，以聚合酶链反应、限制性内切核酸酶检测-4533G/A多态性。
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